Effects of 3,3’,4,4’,5-pentachlorobiphenyl on in vitro oocyte maturation in dusky tripletooth goby, Tridentiger obscurus: an implication of estrogenic potency

Polychlorinated biphenyls (PCBs) are endocrine-disrupting chemicals that mimic estrogen in fish. Among the various PCBs, 3,3’,4,4’,5-pentachlorobiphenyl (PCB126), considered an antagonist, has been reported to elicit estrogenic activity We investigated potency of PCB126 vitro oocyte maturation dusky tripletooth goby, Tridentiger obscurus . In this study, we quantified steroid metabolites following exposure and estradiol-17β (E 2 ). Vitellogenic ovarian follicles were incubated, , with 10 100 ng/mL or E [ 3 H]17α-hydroxyprogesterone as a precursor. Testosterone (T) 2, identified using thin-layer chromatography, high-performance liquid gas chromatography-mass spectrometry. Both increased T metabolite production. Further, vitellogenic exposed (1, ng/mL) (0.01, 0.1 1 from incubation media measured. (100 inhibited production Furthermore, effects on final process. Germinal vesicle migration incubated 0.1, 1, High doses (10 germinal breakdown. These results suggest T. obscurus.